| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Treponema pallidum | CGCAGCAGCAAAGCGTTT | AGGCAACAGCTTCCGCTT | 60.35 | 59.89 | 169 | 64 |
100.00%
|
100.00%
|
| Primer 172 | Treponema pallidum | CGCAGCAGCAAAGCGTTT | ACAGCTTCCGCTTCACCA | 60.35 | 59.17 | 164 | 64 |
100.00%
|
100.00%
|
| Primer 173 | Treponema pallidum | TGCAGTGCAGGTTCCGTT | GCATCGCCTTGCGGTTTT | 59.81 | 59.74 | 254 | 64 |
100.00%
|
100.00%
|
| Primer 174 | Treponema pallidum | TGTAGTGCTCGTTGGTCGG | TGCTCCCAACGGCTTTGA | 59.71 | 59.49 | 243 | 63 |
100.00%
|
100.00%
|
| Primer 175 | Treponema pallidum | TTCGTGTATGGCTGCGCA | CGCATGCGAGGTCCGATAT | 60.05 | 60.08 | 234 | 63 |
100.00%
|
100.00%
|
| Primer 176 | Treponema pallidum | TGCGTGGCTGCACACATA | TGCGCACATGTAGCACCT | 59.97 | 59.65 | 250 | 63 |
100.00%
|
100.00%
|
| Primer 177 | Treponema pallidum | TGCGTGGCTGCACACATA | TGCTGCGCACATGTAGCA | 59.97 | 60.36 | 253 | 63 |
100.00%
|
100.00%
|
| Primer 178 | Treponema pallidum | TTGCACGCAGCTGGTTTC | TGTCGCGTGCACTAAGCT | 59.28 | 59.66 | 246 | 61 |
100.00%
|
100.00%
|
| Primer 179 | Treponema pallidum | ACCGGTGTTGTTAGCGCT | TGCTGCGCACATGTAGCA | 59.58 | 60.36 | 172 | 61 |
100.00%
|
100.00%
|
| Primer 180 | Treponema pallidum | ATGCGCAGGGCTGTTTGA | CATCCCCAAACACTCGGCT | 60.28 | 60.00 | 153 | 61 |
100.00%
|
100.00%
|